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- Title
The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis.
- Authors
Miao Lin; Long Li; Liping Li; Pokhrel, Gaurab; Guisheng Qi; Ruiming Rong; Tongyu Zhu
- Abstract
Background Renal ischemia-reperfusion injury (IRI) increases the rates of acute kidney failure, delayed graft function, and early mortality after kidney transplantation. The pathophysiology involved includes oxidative stress, mitochondrial dysfunction, and immune-mediated injury. The antioxidation, anti-apoptosis, and anti-inflammation properties of baicalin, a flavonoid glycoside isolated from Scutellaria baicalensis, have been verified. This study therefore assessed the effects of baicalin against renal IRI in rats. Methods Baicalin was intraperitoneally injected 30 min before renal ischemia. Serum and kidneys were harvested 24 h after reperfusion. Renal function and histological changes were assessed. Markers of oxidative stress, the Toll-like receptor (TLR)2 and TLR4 signaling pathway, mitochondrial stress, and cell apoptosis were also evaluated. Results Baicalin treatment decreased oxidative stress and histological injury, and improved kidney function, as well as inhibiting proinflammatory responses and tubular apoptosis. Baicalin pretreatment also reduced the expression of TLR2, TLR4, MyD88, p-NF-κB, and p-IκB proteins, as well as decreasing caspase-3 activity and increasing the Bcl-2/Bax ratio. Conclusions Baicalin may attenuate renal ischemia-reperfusion injury by inhibiting proinflammatory responses and mitochondria-mediated apoptosis. These effects are associated with the TLR2/4 signaling pathway and mitochondrial stress.
- Subjects
ANIMAL experimentation; APOPTOSIS; INFLAMMATION; KIDNEYS; RATS; REPERFUSION injury; RESEARCH funding
- Publication
BMC Complementary & Alternative Medicine, 2014, Vol 14, Issue 1, p19
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-14-19