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- Title
Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014.
- Authors
Qiwen Wu; Zhili Li; Guanqun Zhang; Jianqiang Niu; Xiduo Zeng; Baoli Sun; Jingyun Ma
- Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007-2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%-99.7% and 80.9%-100%, respectively. Phylogenetic analysis based on the NSP2 gene showed that highly pathogenic (HP)-PRRSV was still the dominant virus in southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499-510. Phylogenetic analysis based on the ORF5 gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in southern China from 2007 to 2014, suggesting that, in recent years, the type 2 PRRSV was more diverse in southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution and used to study evolutionary relationships among PRRSV strains in China.
- Subjects
PORCINE reproductive &; respiratory syndrome; ENZYME-linked immunosorbent assay; AMINO acids; PHYLOGENY; NUCLEOTIDE sequence
- Publication
Journal of Veterinary Science, 2017, Vol 18, Issue 3, p317
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2017.18.3.317