We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
miR‐208 inhibits myocardial tissues apoptosis in mice with acute myocardial infarction by targeting inhibition of PDCD4.
- Authors
Wang, Qiang; Zhou, Huxiang; Zhu, Xiaobo; Jiang, Feng; Yu, Qiuhua; Zhang, Junjie; Ji, Yaxiang
- Abstract
This article aimed to investigate the role of miR‐208 in the apoptosis of myocardial tissues in acute myocardial infarction (AMI) mice. The AMI mouse model was constructed. Then, miR‐208 expression in AMI mice was regulated by transfection. The mouse myocardial tissues were subject to hematoxylin–eosin (HE) staining, TUNEL assay, and immunofluorescence analysis. H9c2 cell transfection and hypoxia induction were then completed, and cell apoptosis and cytokine levels were tested. Additionally, RNA pull‐down and dual luciferase reporter gene assays were conducted for exploring the relation of miR‐208 with programmed cell death 4 (PDCD4). Additionally, fluorescence in situ hybridization (FISH) was conducted for investigating miR‐208 and PDCD4 colocalization within H9c2 cells. AMI mice had severe damage, apoptosis, decreased miR‐208 expression, increased IL‐1β, IL‐6, IL‐8 levels, whereas reduced IL‐10 level within myocardial tissues. H9c2 cells under hypoxia induction exhibited decreased miR‐208 expression, promoted apoptosis, increased protein expression of Bax and cleaved‐caspase‐3, decreased protein expression of Bcl‐2 and caspase‐3, elevated IL‐1β, IL‐6, IL‐8 levels and decreased IL‐10 level. miR‐208 upregulation alleviated the damage and apoptosis of myocardial tissues in AMI mice. AMI mice with miR‐208 upregulation showed decreased expression of Bax and cleaved‐caspase‐3, increased expression of Bcl‐2 and caspase‐3, reduced levels of IL‐1β, IL‐6, IL‐8, whereas an increased level of IL‐10. miR‐208 showed direct inhibition of PDCD4. PDCD4 and miR‐208 were mainly co‐expressed in the cytoplasm. The upregulated PDCD4 expression abolished miR‐208's suppression of H9c2 cell apoptosis induced by hypoxia. Besides this, miR‐208 inhibited myocardial tissue apoptosis in AMI mice by inhibiting PDCD4 expression.
- Subjects
AMERICAN Megatrends Inc.; MYOCARDIAL infarction; FLUORESCENCE in situ hybridization; APOPTOSIS; BCL-2 proteins; MICE
- Publication
Journal of Biochemical & Molecular Toxicology, 2022, Vol 36, Issue 12, p1
- ISSN
1095-6670
- Publication type
Article
- DOI
10.1002/jbt.23202