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- Title
Determination for KIR genotype and allele copy number via real-time quantitative PCR method.
- Authors
Tao, Sudan; You, Xuan; Wang, Jielin; Zhang, Wei; He, Ji; Zhu, Faming
- Abstract
Killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) play crucial roles in regulating NK cell activity. Here, we report a real-time quantitative PCR (qPCR) to genotype all KIR genes and their copy numbers simultaneously. With 18 pairs of locus-specific primers, we identified KIR genes by Ct values and determined KIR copy number using the 2−∆Ct method. Haplotypes were assigned based on KIR gene copy numbers. The real-time qPCR results were consistent with the NGS method, except for one sample with KIR2DL5 discrepancy. qPCR is a multiplex method that can identify KIR copy number, which helps obtain a relatively accurate haplotype structure, facilitating increased KIR research in laboratories where NGS or other high-resolution methods are not available.
- Subjects
KILLER cell receptors; GENOTYPES; ALLELES; HLA histocompatibility antigens; HAPLOTYPES; KILLER cells
- Publication
Immunogenetics, 2024, Vol 76, Issue 2, p137
- ISSN
0093-7711
- Publication type
Article
- DOI
10.1007/s00251-023-01331-7