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- Title
Effects of intermittent hypoxia training on leukocyte pyruvate dehydrogenase kinase 1 (PDK-1) mRNA expression and blood insulin level in prediabetes patients.
- Authors
Serebrovska, Tetiana V.; Portnychenko, Alla G.; Portnichenko, Vladimir I.; Xi, Lei; Egorov, Egor; Antoniuk-Shcheglova, Ivanna; Naskalova, Svitlana; Shatylo, Valeriy B.
- Abstract
<bold>Purpose: </bold>Intermittent hypoxia training/treatment (IHT) is an emerging therapeutic approach to alleviate chronic diseases, such as diabetes. The present study investigated the effects of IHT on blood leucocyte pyruvate dehydrogenase kinase 1 (PDK-1) mRNA expression and its relationship with the changes in blood insulin level.<bold>Methods: </bold>Seven adult healthy volunteers and 11 prediabetic patients participated in this study. A 3-week course of IHT consisted of a 40-min session of 4 cycles of 5-min 12% O2 and 5-min room air breathing per day, 3 sessions per week for 3 weeks (i.e., total 9 sessions of IHT). Plasma insulin levels and leukocyte PDK-1 mRNA expression were determined at various time points either under fasting condition or following oral glucose tolerance test (OGTT). Correlation between the IHT-induced changes in PDK-1 mRNA and insulin or glucose levels in the same serological samples was analyzed.<bold>Results: </bold>At pre-IHT baseline, PDK-1 mRNA expression was two times higher in prediabetes than control subjects. IHT resulted in significant augmentation in PDK-1 mRNA expression (> twofold) in prediabetes at the end of 3-week IHT and remained elevated 1 month after IHT, which was correlated with a significantly reduced insulin release and lower blood glucose after glucose loading with OGTT.<bold>Conclusion: </bold>IHT can trigger beneficial effects in normalizing blood insulin levels in prediabetic patients under oral glucose load, which were closely correlated with an enhanced mRNA expression of PDK-1 in leukocytes. Further clinical trials are warranted to validate the utility of IHT as a non-invasive complementary therapy against diabetes-associated pathologies.
- Subjects
PYRUVATE dehydrogenase kinase; INTERVAL training; HYPOXIA-inducible factor 1; INSULIN; GLUCOSE tolerance tests; MESSENGER RNA; PHYSIOLOGICAL adaptation; LEUCOCYTES; PREDIABETIC state; RNA; TRANSFERASES
- Publication
European Journal of Applied Physiology, 2019, Vol 119, Issue 3, p813
- ISSN
1439-6319
- Publication type
journal article
- DOI
10.1007/s00421-019-04072-2