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- Title
The Role of Ethylene Diisothiocyanate (EDI) in the Antifungal Action of Disodium Ethylene Bisdithiocarbamate (Nabam). I. The Mode of Action of EDI on the Metabolism of Yeast.
- Authors
Håbrekke, J. I.; Goksøyr, J.
- Abstract
With glucose as a substrate, the oxygen consumption in yeast is inhibited by 2.10-5 M ethylene diisothiocyanate. The degree of inhibition was only to a small extent dependent on pH. Radiorespirometric experiments with uniformely labelled glucose showed that the CO2-production from glucose increased, probably due to increased glycolytic activity. Conversion of C-1 to CO2 was unaffected by the inhibitor, while the evolution of CO2 from C-6 was strongly inhibited. The same was the case with CO2 from C-1 in acetate. Respiration of ethanol was more strongly inhibited than that of glucose or acetate. Experiments with dual wavelength spectrophotometry showed the inhibition to be located on the Krebs cycle side of the respiratory flavoproteins. It is concluded that the action of ethylene diisothiocyanate on respiration must be located at the mitochondria.
- Subjects
MONOSACCHARIDES; EDIBLE fungi; YEAST-free diet; RESPIRATION; PHOTOSYNTHETIC oxygen evolution; KREBS cycle
- Publication
Physiologia Plantarum, 1970, Vol 23, Issue 3, p517
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1970.tb06443.x