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- Title
DIFFERENTIAL DEVELOPMENT OF CARBACHOL-INDUCED DESENSITIZATION IN RECEPTOR-MEDIATED Ca<sup>2+</sup> INFLUX AND Ca<sup>2+</sup> RELEASE PATHWAYS IN SMOOTH MUSCLE OF GUINEA-PIG TAENIA CAECI.
- Authors
Hishinuma, Shigeru; Matsumoto, Yukio; Sato, Ryo; Saito, Masaki
- Abstract
1. We have found that development of carbachol (CCh)-induced desensitization to receptor agonists, but not to receptor by-passed stimulation, is transiently interrupted by a Ca2+-dependent resensitization during the early stage in the smooth muscle of guinea-pig taenia caeci. To further characterize the receptor-mediated signal transduction pathways involved in this peculiar desensitization process, we examined the desensitization processes during Ca2+ influx- and Ca2+ release-mediated contractions in response to activation of muscarinic receptors or histamine H1 receptors. 2. Desensitization treatment with 10−4 mol/L CCh for 30 min in the presence of extracellular Ca2+ resulted in desensitization to the muscarinic agonists McN-A-343 or AHR-602, which are known to induce contraction only in the presence of extracellular Ca2+ in taenia caeci. The development of desensitization to these agonists was interrupted by a transient resensitization at 1 min. In contrast, the transient resensitization phase was lost following removal of extracellular Ca2+ during the desensitization treatment with CCh; under these conditions, the desensitization developed gradually without an apparent resensitization phase. 3. Contractions to 10−4 mol/L CCh and 10−4 mol/L histamine in the absence of extracellular Ca2+ were gradually desensitized without a resensitization phase following the CCh desensitization treatment, irrespective of the presence or absence of extracellular Ca2+ during CCh treatment, although the onset of the desensitization was delayed under Ca2+-free conditions. 4. These results suggest that the receptor-mediated Ca2+ influx and Ca2+ release pathways are differentially desensitized to CCh and that the transient resensitization appears to regulate the desensitization process in response to Ca2+ influx-mediated contraction. Such differential processes of desensitization in receptor-mediated bifurcated signalling pathways may determine cellular responsiveness to certain types of stimuli, depending on the different Ca2+ sources required for contraction.
- Subjects
SMOOTH muscle; MUSCARINIC receptors; RECEPTOR antibodies; HISTAMINE receptors; GUINEA pigs; ANIMAL models in research
- Publication
Clinical & Experimental Pharmacology & Physiology, 2007, Vol 34, Issue 3, p191
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/j.1440-1681.2007.04571.x