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- Title
In Vivo Imaging of Lymph Node Migration of MNP- and In-Labeled Dendritic Cells in a Transgenic Mouse Model of Breast Cancer (MMTV- Ras).
- Authors
Martelli, Cristina; Borelli, Manuela; Ottobrini, Luisa; Rainone, Veronica; Degrassi, Anna; Russo, Micaela; Gianelli, Umberto; Bosari, Silvano; Fiorini, Carlo; Trabattoni, Daria; Clerici, Mario; Lucignani, Giovanni
- Abstract
Purpose: The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model. Procedures: Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV- Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data. Results: DC labeling, both with MNPs and with In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs. Conclusions: MRI and SPECT imaging , by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
- Subjects
MEDICAL imaging systems; DIAGNOSTIC imaging; TUMORS; BREAST cancer; DENDRITIC cells; MICE
- Publication
Molecular Imaging & Biology, 2012, Vol 14, Issue 2, p183
- ISSN
1536-1632
- Publication type
Article
- DOI
10.1007/s11307-011-0496-0