We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Conditional Control of CRISPR/Cas9 Function.
- Authors
Zhou, Wenyuan; Deiters, Alexander
- Abstract
The recently discovered CRISPR/Cas9 endonuclease system, comprised of a guide RNA for the recognition of a DNA target and the Cas9 nuclease protein for binding and processing the target, has been extensively studied and has been widely applied in genome editing, synthetic biology, and transcriptional modulation in cells and animals. Toward more precise genomic modification and further expansion of the CRISPR/Cas9 system as a spatiotemporally controlled gene regulatory system, several approaches of conditional activation of Cas9 function using small molecules and light have recently been developed. These methods have led to improvements in the genome editing specificity of the CRISPR/Cas9 system and enabled its activation with temporal and spatial precision.
- Subjects
ENDONUCLEASES; SYNTHETIC biology; CRISPRS; GENOMES; PROTEIN binding
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 18, p5394
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201511441