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- Title
Over 10 000 Peptide Identifications from the HeLa Proteome by Using Single-Shot Capillary Zone Electrophoresis Combined with Tandem Mass Spectrometry.
- Authors
Sun, Liangliang; Hebert, Alexander S.; Yan, Xiaojing; Zhao, Yimeng; Westphall, Michael S.; Rush, Matthew J. P.; Zhu, Guijie; Champion, Matthew M.; Coon, Joshua J.; Dovichi, Norman J.
- Abstract
Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance for this analysis has so far fallen far below that of reversed-phase liquid chromatography (RPLC)-MS/MS. The use of a CZE method with a wide separation window (up to 90 min) and high peak capacity (ca. 300) is reported. This method was coupled to an Orbitrap Fusion mass spectrometer through an electrokinetically pumped sheath-flow interface for the analysis of complex proteome digests. Single-shot CZE-MS/MS lead to the identification of over 10 000 peptides and 2100 proteins from a HeLa cell proteome digest in approximately 100 min. This performance is nearly an order of magnitude better than earlier CZE studies and is within a factor of two to four of the state-of-the-art nano ultrahigh-pressure LC system.
- Subjects
HELA cells; PEPTIDE analysis; PROTEOMICS; CAPILLARY electrophoresis; TANDEM mass spectrometry
- Publication
Angewandte Chemie International Edition, 2014, Vol 53, Issue 50, p13931
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201409075