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- Title
Molecular study of 2019 dengue fever outbreaks in Nepal.
- Authors
Poudyal, Pranita; Sharma, Kesari; Dumre, Shyam Prakash; Bastola, Anup; Chalise, Bimal Sharma; Shrestha, Bikesh; Poudel, Anubhav; Giri, Anita; Bhandari, Paramanda; Shah, Yogendra; Poudel, Ram Chandra; Khadka, Deegendra; Maharjan, Jyoti; Tun, Mya Myat Ngwe; Morita, Kouchi; Pandey, Basu Dev; Pandey, Kishor
- Abstract
Background Dengue cases have been continuously reported in Nepal, including some large outbreaks, since its first introduction in 2004. The disease is now expanding towards newer locations above 1400 m high, especially the country's capital city, Kathmandu. In 2019, >14,000 dengue cases including six deaths were reported. This study was aimed at the detection and molecular characterization of dengue virus (DENV) in dengue patients. Methods A total of 451 patients were enrolled in this study. Demographic, clinical and laboratory information was collected from dengue patients. Dengue infection was confirmed by antibody/antigen detection assays followed by RT-PCR analysis. Results The DENV patients showed fever, body ache, headache, myalgia, retro-orbital pain and arthralgia. The platelets were decreased, serum liver enzymes were increased and leucopenia was seen. Out of 195 patients, 111 (57.0%) were positive for DENV RNA by consensus PCR. We found DENV-2, 70 (63.1%) as the predominant serotype responsible for the 2019 outbreak, while DENV-3 was detected in two patients. Conclusion Our findings suggest that DENV-2 was the major serotype causing the 2019 massive outbreak in Nepal. This information will help in disease control programs to understand the molecular epidemiology and its changing trend.
- Subjects
NEPAL; KATHMANDU (Nepal); DENGUE hemorrhagic fever; DENGUE; DENGUE viruses; LIVER enzymes; MOLECULAR epidemiology; DEATH rate
- Publication
Transactions of the Royal Society of Tropical Medicine & Hygiene, 2021, Vol 115, Issue 6, p619
- ISSN
0035-9203
- Publication type
Article
- DOI
10.1093/trstmh/traa096