We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
DermaVir: A Novel Topical Vaccine for HIV/AIDS.
- Authors
Lisziewicz, Julianna; Trocio, Jeffrey; Whitman, Lucia; Varga, Georg; Xu, Jianqing; Bakare, Nyasha; Erbacher, Patrick; Fox, Cecil; Woodward, Ruth; Markham, Phil; Arya, Suresh; Behr, Jean-Paul; Lori, Franco
- Abstract
Human immunodeficiency virus (HIV) vaccines have the potential to improve antiretroviral drug treatment by inducing cytotoxic killing of HIV-infected cells. Prophylactic vaccines utilize new antigens to initiate immunity; however, in HIV-infected individuals the load of viral antigen is not the limiting factor for the restoration of immune responses. Here we describe a novel immunization strategy with DermaVir that improves viral antigen presentation using dendritic cells (DC). DermaVir contains a distinctive plasmid DNA expressing all HIV proteins except integrase to induce immune responses with broad specificity. The DNA is formulated to a mannosilated particle to target antigen-presenting cells and to protect the DNA from intracellular degradation. After topical application, DermaVir-transduced cells migrate from the skin to the draining lymph node and interdigitate as DermaVir-expressing, antigen-presenting DC. We compared the immunogenicity of topical andex vivoDC-based DermaVir vaccinations in naïve rhesus macaques. Both vaccinations induced simian immunodeficiency virus-specific CD4 helper and CD8 memory T cells detected by anin vivoskin test and anin vitrointracellular cytokine-based assay. Topical DermaVir vaccination represents an improvement upon existingex vivoDC-based immunization technologies and may provide a new therapeutic option for HIV-infected patients.
- Subjects
ANTIRETROVIRAL agents; HIV; AIDS; HIV-positive persons; DENDRITIC cells; DNA
- Publication
Journal of Investigative Dermatology, 2005, Vol 124, Issue 1, p160
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/j.0022-202X.2004.23535.x