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- Title
Processing of hematopoietic stem cells from peripheral blood before cryopreservation: use of a closed automated system.
- Authors
Zinno, Francesco; Landi, Fabiola; Scerpa, Maria Cristina; Aureli, Viviana; Lanti, Alessandro; Ceccarelli, Stefano; Caniglia, Maurizio; Miele, Massimino Jan; Daniele, Nicola; Landolfo, Attilio; Cometa, Angela Maria; Locatelli, Franco; Isacchi, Giancarlo
- Abstract
BACKGROUND: Hematopoietic stem cell transplantation is commonly used to treat several oncohematologic diseases. The autologous hematopoietic progenitor cells collected through apheresis (HPC-A) must be cryopreserved and stored before use in vivo. Cell processing that precedes cryopreservation of HPC-A includes volume reduction aimed at reducing the amount of dimethyl sulfoxide used, as well as storage space. STUDY DESIGN AND METHODS: The aim of our study was to assess the effectiveness of volume reduction performed with an automated closed system, namely, the Sepax S100 cell separation device (Biosafe SA). A total of 165 procedures were carried out on concentrates collected from 104 adult and pediatric patients. As a control group, 30 HPC-A units processed according to the standard method (i.e., centrifugation at a speed of 850 × g for 10 minutes, followed by manual plasma reduction) were evaluated. RESULTS: The volume reduction obtained was 59% (range, 20.54%-84.21%; standard deviation [SD], ±12.19%), going from 236 mL (range, 100-443 mL; SD, ±80.41 mL) to 97 mL (range, 33.00-263.00 mL; SD, ±47.41 mL); recovery of nucleated cells was 90% (range, 64.84%-105.93%; SD, ±8.76%), while that of CD34+ cells was 91% (range, 59.30%-119.37%; SD, ±13.30%). These values did not differ from those obtained using the standard method. Automated processing required 20 minutes versus 40 minutes of manual processing. DISCUSSION: Our data demonstrate that volume reduction carried out with the Sepax S100 automated system was particularly effective; cell recovery was excellent and the time spent was short. Moreover, the closed system allows cell processing to be carried out in a contamination-controlled environment, in accordance with good manufacturing practice guidelines.
- Subjects
HEMATOPOIETIC stem cell transplantation; CRYOPRESERVATION of organs, tissues, etc.; HEMAPHERESIS; DIMETHYL sulfoxide; CELL separation
- Publication
Transfusion, 2011, Vol 51, Issue 12, p2656
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.1537-2995.2011.03180.x