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- Title
RHCE*ceCF encodes partial c and partial e but not CELO, an antigen antithetical to Crawford.
- Authors
Hipsky CH; Lomas-Francis C; Fuchisawa A; Reid ME; Moulds M; Christensen J; Nickle P; Vege S; Westhoff C; Hipsky, Christine Halter; Lomas-Francis, Christine; Fuchisawa, Akiko; Reid, Marion E; Moulds, Marilyn; Christensen, Joann; Nickle, Pam; Vege, Sunitha; Westhoff, Connie
- Abstract
<bold>Background: </bold>RH43 (Crawford) is encoded by RHCE*ce with nucleotide changes 48G>C, 697C>G, and 733C>G (RHCE*ceCF). We investigated the Rh antigen expression and antibody specificities in four patients with this allele.<bold>Study Design and Methods: </bold>Hemagglutination tests, DNA extraction, polymerase chain reaction (PCR)-restriction fragment length polymorphism, allele-specific PCR, reticulocyte RNA isolation, reverse transcription-PCR cDNA analyses, cloning, and sequencing were performed by standard procedures.<bold>Results: </bold>Red blood cells (RBCs) from two patients typed D+C-E-c+e+/-, hrS-/+W, hrB- and their serum was reactive (3+) with all RBC samples of common Rh phenotype tested, but nonreactive with Rhnull or D-- RBCs (apparent alloanti-Rh17). At the RHCE locus, Patient 1 was homozygous for RHCE*ceCF, and Patient 2 inherited RHCE*ceCF in trans to a silenced RHCE*cE. Cross-testing of serum and RBCs from these two samples showed mutual compatibility, indicating that both antibodies define the same novel high-prevalence antigen on Rhce. Two additional patients, one whose serum contained alloanti-c but the RBCs typed C+c+ and one whose serum contained anti-e but the RBCs typed E+e+, also had RHCE*ceCF. RHCE*Ce was present in trans in the former and RHCE*cE in the latter patient.<bold>Conclusion: </bold>We report that amino acid changes on RhceCF (Trp16Cys, Gln233Glu, and Leu245Val) alter the protein to the extent that c and e antigens are partial, and a high-prevalence antigen, we have named CELO (provisional ISBT Number 004058; RH58) is not expressed. CELO is antithetical to RH43 (Crawford).
- Publication
Transfusion, 2011, Vol 51, Issue 1, p25
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/j.1537-2995.2010.02764.x