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- Title
Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay and mass spectrometry; relevance for the diagnostics in aging men.
- Authors
Huhtaniemi, Ilpo T.; Tajar, Abdelouahid; Lee, David M.; O'Neill, Terence W.; Finn, Joseph D.; Bartfai, György; Boonen, Steven; Casanueva, Felipe F.; Giwercman, Aleksander; Thang S. Han; Kula, Krzysztof; Labrie, Fernand; Lean, Michael E. J.; Pendleton, Neil; Punab, Margus; Silman, Alan J.; Vanderschueren, Dirk; Forti, Gianni; Wu, Frederick C. W.
- Abstract
Background: The limitations of serum testosterone and estradiol (E2) measurements using nonextraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce. Methods:We compared serumtestosterone and E2measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)-MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (nZ3174; age 40-79 years), peripheral serum testosterone and E2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC-MS methods. Results: Over a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (RZ0.93, P!0.001), which was less robust in the hypogonadal range (!11 nmol/l; RZ0.72, P!0.001). The IA/MS correlation was weaker in E2 measurements (RZ0.32, P!0.001, at E2 !40.8 pmol/l, and RZ0.74, P!0.001, at E2 O40.8 pmol/l). Using MS as the comparator method, IAascertained lowtestosterone compatible with hypogonadism(!11 nmol/l),with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E2 (!40.7 pmol/l) were 13.3 and 99.3%, and for high E2 (O120 pmol/l) 88.4 and 88.6%. Conclusion: A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis ofmale hypogonadism. The IAused for E2 measurements showed poor correlation with MS and may only be suitable for the detection of high E2 in men.
- Subjects
COMPARATIVE studies; BLOOD serum analysis; TESTOSTERONE; ESTRADIOL; EUROPEANS; IMMUNOASSAY; MASS spectrometry; GAS chromatography
- Publication
European Journal of Endocrinology, 2012, Vol 166, Issue 6, p983
- ISSN
0804-4643
- Publication type
Article
- DOI
10.1530/EJE-11-1051