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- Title
The expression of 11β-hydroxysteroid dehydrogenase type 1 is increased in experimental periodontitis in rats.
- Authors
Takaya Nakata; Makoto Umeda; Hiroaki Masuzaki; Hirofumi Sawai
- Abstract
Background: The involvement of 11 β-hydroxysteroid dehydrogenase type 1 (11 β-HSD1), which converts inactive glucocorticoids into active glucocorticoids intracellularly, in metabolic diseases and chronic inflammatory diseases has been elucidated. We recently reported that an increase in 11 β-HSD1 expression was associated with chronic periodontitis in humans irrespective of obesity. To further clarify the role of 11β-HSD1 in chronic periodontitis, the expression of 11β-HSD1 was investigated in experimental periodontitis model in rats. Methods: Experimental periodontitis was induced by silk ligature of left maxillary second molars of 7-week-old male Wistar rats, and periodontal tissues were collected at day 3. The expression of 11β-HSD1, 11β-HSD2, and TNFα mRNA was examined using real time reverse transcription-polymerase chain reaction. The expression of TNFα was used as an indicator of inflammation. Thus, the rats in which the levels of TNFα mRNA were increased in the ligature-induced periodontitis compared with the control were analysed. Results: The findings demonstrated that the expression of 11β-HSD1 mRNA was significantly increased in experimental periodontitis compared with the control. The increase in the levels of 11β-HSD1 mRNA in the ligature-induced periodontitis compared with the control was positively correlated with that of TNFα mRNA. On the other hand, the expression of 11β-HSD2 mRNA, which inactivates glucocorticoids, was slightly decreased in experimental periodontitis. Therefore, the ratio of 11 β-HSD1 versus 11 β-HSD2 mRNA was significantly higher in experimental periodontitis than in the control. Conclusions: These results suggest that the increased expression of 11 β-HSD1, which would result in the increased levels of intracellular glucocorticoids, may play a role in the pathophysiology of experimental periodontitis.
- Subjects
ANIMAL experimentation; COMPARATIVE studies; GLUCOCORTICOIDS; INFLAMMATION; OXIDOREDUCTASES; PERIODONTITIS; POLYMERASE chain reaction; RATS; RNA; TUMOR necrosis factors; REVERSE transcriptase polymerase chain reaction
- Publication
BMC Oral Health, 2016, Vol 16, p1
- ISSN
1472-6831
- Publication type
Article
- DOI
10.1186/s12903-016-0303-z