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- Title
Substrate-induced remodeling of the active site regulates human HTRA1 activity.
- Authors
Truebestein, Linda; Tennstaedt, Annette; Mönig, Timon; Krojer, Tobias; Canellas, Flavia; Kaiser, Markus; Clausen, Tim; Ehrmann, Michael
- Abstract
Crystal structures of active and inactive conformations of the human serine protease HTRA1 reveal that substrate binding to the active site is sufficient to stimulate proteolytic activity. HTRA1 attaches to liposomes, digests misfolded proteins into defined fragments and undergoes substrate-mediated oligomer conversion. In contrast to those of other serine proteases, the PDZ domain of HTRA1 is dispensable for activation or lipid attachment, indicative of different underlying mechanistic features.
- Subjects
PROTEIN conformation; SERINE proteinases; PROTEOLYSIS; LIPOSOMES; PROTEOLYTIC enzymes
- Publication
Nature Structural & Molecular Biology, 2011, Vol 18, Issue 3, p386
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2013