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- Title
Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition.
- Authors
Kumar, Parveen; Verma, Arpana; Kumar, Manish; De, Sachinandan; Kumar, Rakesh; Datta, Tirtha
- Abstract
Purpose: This study evaluates the effect of low oxygen conditions (5 Vs 20 %) on buffalo embryo development. Expression patterns of key glucose metabolism genes ( HK, PFK, LDH, PDH, G6PDH and Glut1) were assessed in buffalo oocytes and embryos cultured at 5 and 20 % oxygen and correlated with development rate. Methods: Maturation rate was observed by determining MII stages by Aceto-orcein method and blastocyst formation was observed at 7 day post insemination (dpi). Expression levels of genes were determined by real time PCR in oocytes / embryos at 5 and 20 % O. Results: Oocyte maturation and blastocyst formation rates were significantly higher at 5 % O as compared to 20 % O ( P < 0.05). The expression pattern of glycolytic genes ( HK, PFK and G6PDH) indicated that oocytes and embryos under 5 % O tend to follow anaerobic glycolysis and pentose phosphate pathways to support optimum embryo development. Under 20 % O, oocytes and embryos had high expression of PDH indicating higher oxidative phosphorylation. Further, less G6PDH expression at 20 % O was indicative of lower pentose phosphate activity. Higher expression of LDH was observed in oocytes and embryos under 20 % O indicating sub-optimal culture conditions. High Glut1 activity was observed in the oocytes / embryos at 5 % O, indicative of high glucose uptake correlating with high expression of glycolytic genes. Conclusion: The expression patterns of glucose metabolism genes could be a valuable indicator of the development potential of oocytes and embryos. The study indicates the importance of reduced oxygen conditions for production of good quality embryos.
- Subjects
CATTLE embryos; GLUCOSE metabolism; GENE expression; CATTLE reproduction; OVUM
- Publication
Journal of Assisted Reproduction & Genetics, 2015, Vol 32, Issue 3, p471
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-014-0418-6