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- Title
Different effects of omega-3 fatty acids on the cell cycle in C2C12 myoblast proliferation.
- Authors
Peng, Yunqian; Zheng, Yu; Zhang, Yunsheng; Zhao, Jie; Chang, Fei; Lu, Tianyu; Zhang, Ran; Li, Qiuyan; Hu, Xiaoxiang; Li, Ning
- Abstract
Polyunsaturated fatty acids (PUFAs) are important molecules for human health. We investigated the effects of three major omega-3 PUFAs on C2C12 myoblast proliferation. Both docosahexaenoic (DHA) and eicosapentaenoic (EPA) acids decreased cell growth, whereas linolenic (ALA) acid did not, compared with the control. Cell cycle analysis showed that G phase duration was increased markedly and S-phase duration was decreased by DHA and EPA. In contrast, there was no change in the G or S-phase duration when the cells were treated with linolenic acid. To determine how DHA and EPA affected the cell cycle, cyclins and MAPK proteins were investigated. Western blotting and real-time quantitative PCR showed that DHA and EPA decreased cyclin E and CDK2 levels at both the protein and mRNA level. Also, MAPK phosphorylation levels were decreased by treatment with DHA and EPA. Our results indicated that different kinds of n-3 PUFA differentially affected myoblast cell proliferation. DHA and EPA decreased skeletal muscle cell proliferation through a mechanism involving MAPK-ERK.
- Subjects
OMEGA-3 fatty acids; MYOBLASTS; CELL proliferation; CYCLIN E; MESSENGER RNA
- Publication
Molecular & Cellular Biochemistry, 2012, Vol 367, Issue 1/2, p165
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-012-1329-4