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- Title
Integrated Analysis of Copy Number Alterations and Loss of Heterozygosity in Human Pancreatic Cancer Using a High-Resolution, Single Nucleotide Polymorphism Array.
- Authors
Lian-Jie Lin; Asaoka, Yoshinari; Tada, Motohisa; Sanada, Masashi; Nannya, Yasuhito; Tanaka, Yasuo; Tateishi, Keisuke; Ohta, Miki; Seto, Motoko; Sasahira, Naoki; Tada, Minoru; Kawabe, Takao; Chang-Qing Zheng; Kanai, Fumihiko; Ogawa, Seishi; Omata, Masao
- Abstract
Objective: To chart molecular genetic events in pancreatic cancer. Methods: We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism (SNP) array. We verified the data using genomic PCR and applied them to clinical samples. Results: Twenty-six homozygous deletion regions were detected in at least 1 cell line and LOH was found at 9p, 18q, 17p, 8p, 13q, 6q, 3p, 6p, 22q, 9q and 12q with high frequency (>50%), consistent with a previous study. Moreover, we found 23 amplified regions in at least 2 cell lines, including 8 unreported loci. We then examined representative genes at the 8 amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens. Conclusion: Using high-resolution SNP arrays, we studied genome-wide copy number alterations and LOH simultaneously. We identified several novel and minute genomic amplifications, which contained candidate oncogenes in human pancreatic cancers. Copyright © 2008 S. Karger AG, Basel
- Subjects
MOLECULAR genetics; PANCREATIC cancer; HETEROZYGOSITY; GENETIC polymorphisms; CELL lines
- Publication
Oncology, 2008, Vol 75, Issue 1/2, p102
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000155813