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- Title
Oxidative damage of hydrogen peroxide to boar sperm.
- Authors
Lin, F. F.; Wang, S. C.; Chen, M. C.
- Abstract
The sensitivity of boar sperm to oxidative stress is variable, which affects the preservation of sperm. The purpose of this study was to analyze the effect of different concentrations of hydrogen peroxide (H2O2) on boar sperm quality. Boar semen was treated with 0, 250, 500, 750 and 1,000 pM H2O2 for 1 hour to determine the sperm motility. In the 0 and 1000 pM groups, the sperm viability, mitochondrial activity and acrosome integrity were also analyzed. The results showed that as the concentration of H2O2 increased, the sperm motility decreased significantly (P < 0.05). Compared with the control group (0 µM H2O2), the sperm viability and mitochondrial activity of boar semen induced by high concentrations H2O2 significantly decreased (P < 0.05), but had no effect on the acrosome integrity. The mean and variation coefficient of sperm motility of semen treated with 500 pM H2O2 were 57.5 ± 17.7% and 30.8%, and those of the control group were 97.5 ± 1.7% and 30.8%. In summary, the boar semen-induced oxidation by hydrogen peroxide reduced the sperm quality, and 500 µM H2O2 increased the variability of sperm motility.
- Subjects
BOARS; HYDROGEN peroxide; OXIDATIVE stress; SPERM motility; CONTROL groups; ACROSOMES
- Publication
Journal of the Chinese Society of Animal Science, 2021, Vol 50, p156
- ISSN
0253-9187
- Publication type
Article