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- Title
Comparison of pulsed-field gel electrophoresis and three rep-PCR methods for evaluating the genetic relatedness of Stenotrophomonas maltophilia isolates.
- Authors
Lin, C.-W.; Chiou, C.-S.; Chang, Y.-C.; Yang, T.-C.
- Abstract
Aims: In this study, three facile repetitive-sequence PCR (rep-PCR) techniques have been compared with the pulsed-field gel electrophoresis (PFGE) method for differentiating the genetic relatedness of clinical Stenotrophomonas maltophilia isolates. Methods and Results: The dendrograms of 20 S. maltophilia isolates were constructed based on the data obtained from PFGE and three PCR-based methods, i.e. enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), BOX-PCR and repetitive extragenic palindromic-PCR (REP-PCR). When compared with PFGE, ERIC-PCR displayed a much lower discriminatory power, whereas BOX-PCR and REP-PCR had a comparable discriminatory power for close genetic-related isolates. Conclusion: BOX-PCR and REP-PCR can be convenient and effective methods for evaluating the close genetic relatedness of clinical S. maltophilia isolates. Significance and Impact of the Study: A rapid method for determining S. maltophilia’s close genetic relatedness provides a convenient tool for understanding the epidemiology of S. maltophilia.
- Subjects
ELECTROPHORESIS; GENETICS; POLYMERASE chain reaction; EPIDEMIOLOGY; GEL electrophoresis
- Publication
Letters in Applied Microbiology, 2008, Vol 47, Issue 5, p393
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2008.02443.x