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- Title
CircRNA EZH2通过调控miR-30c-5p促进前列腺癌细胞增殖和迁移.
- Authors
赵斌; 段元鹏; 张国颖; 毕城伟; 杨李波; 施致裕; 杨勇; 张建朋1,高婷
- Abstract
Objective To investigate the effect and related mechanism of circRNA EZH2 on proliferation and migration of prostate cancer cells through miR-30c-5p. Methods The expression difference and survival probability of EZH2 in prostate cancer were analyzed according to TCGA database. SiRNA were transfected to silence EZH2 in prostate cancer LNCaP cells, and proliferation and migration of LNCaP cells were detected by CCK-8 assay and wound healing assay, and the expression of miR-30c-5p, JAK1 and PI3K proteins were determined by RT-qPCR and western blot. Results The expression of EZH2 was up-regulated in prostate cancer tissues, and the survival rate of patients with high expression of EZH2 decreased. Compare with normal LNCaP cells, the proliferation and migration of EZH2-silenced LNCaP cells were decreased, miR-30c-5p expression was increased, JAK1 expression was decreased, and PI3K signal was inhibited. Conclusion EZH2 is highly expressed in prostate cancer and can promote the proliferation and migration of LNCaP cells by regulating miR-30c-5p/JAK1 axis and acting on PI3K signaling pathway.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2022, Vol 43, Issue 7, p25
- ISSN
2095-610X
- Publication type
Article
- DOI
10.12259/j.issn.2095-610X.S20220731