We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells.
- Authors
Wei, ChENg; ChEN, Chuanrong; ChENg, Yuxin; Zhu, Lin; Wang, Yu; Luo, Can; He, Yang; Yang, Zhiming; Ji, Zhaoning
- Abstract
Ailanthone, which is extracted from the traditional Chinese medicinal plant Ailanthus altissima, has been thoroughly demonstrated to have anti‑tumor, anti‑HIV, anti‑inflammatory, anti‑malarial, anti‑allergic and anti‑microbial activities. However, the anti‑proliferative effects of ailanthone on HL‑60 cells and potential mechanisms underlying those effects have not been reported. In the present study, we demonstrated the potent cytotoxicity of ailanthone against HL‑60 cells. Annexin V‑APC/7‑ADD staining assay indicated that ailanthone increased the number of apoptotic cells in a dose‑dependent manner. PI staining showed that ailanthone increased the percentage of G0/G1‑phase cells in a dose‑dependent manner. Acridine orange staining suggested that ailanthone induced the formation of acidic vesicular organelles in HL‑60 cells and pretreatment with BaF‑A1 could attenuate this process. Western blotting showed that ailanthone up‑regulated the protein expression levels of beclin‑1 and LC3‑II and down‑regulated those of LC3‑I and p62 in a dose‑dependent manner. Use of BaF‑A1 showed that the anti‑proliferative effects of ailanthone on HL‑60 cells may be partly attributable to the induction of autophagy‑mediated apoptosis by MTT assay and annexin V‑APC/7‑ADD staining assay.
- Subjects
LEUKEMIA treatment; AUTOPHAGY; CELL death; CHINESE medicine; THERAPEUTIC use of plant extracts
- Publication
Oncology Letters, 2018, Vol 16, Issue 3, p3569
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2018.9101