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- Title
In vitro effects of concentrated growth factors (CGF) on human SH-SY5Y neuronal cells.
- Authors
BORSANI, E.; BUFFOLI, B.; BONAZZA, V.; BRUNELLI, G.; MONINI, L.; INCHINGOLO, F.; BALLINI, A.; REZZANI, R.; RODELLA, L. F.
- Abstract
OBJECTIVE: The aim of this study was to test the in vitro differentiation effects of concentrated growth factors (CGF), a platelet rich preparation, using SH-SY5Y cells, derived from human neuroblastoma. MATERIALS AND METHODS: SH-SY5Y cells were cultured in presence of CGF or retinoic acid (RA). After 72 h of treatment, different parameters were investigated: cell proliferation by an automated cell counter; cell viability by thiazolyl blue tetrazolium bromide (MTT) assay; cell differentiation markers, i.e., neuronal nuclear antigen (NeuN), synaptophysin (SYP) and ß3-tubulin, by immunocytochemistry and Western blotting techniques; release of nerve growth factor (NGF) and brain-derived growth factor (BDNF) by enzyme-linked immunosorbent assay (ELISA) and neurite outgrowth by a dedicated image software. RESULTS: In presence of CGF, the cell proliferation rate and viability decreased, as expected for differentiated SH-SY5Y cells. On the contrary, the cellular differentiation markers increased their expression together with the release of growth factors. Moreover, the neurite outgrowth was improved. CONCLUSIONS: The data suggest that CGF treatment positively affects the cell differentiation, regulating the expression of neuronal markers, the release of growth factors and the neurite length. Taken together these results seem to be promising in the development of new approaches for neural regeneration.
- Subjects
GROWTH factors; NEUROTROPHINS; ENZYME-linked immunosorbent assay; NERVOUS system regeneration; CELL differentiation
- Publication
European Review for Medical & Pharmacological Sciences, 2020, Vol 24, Issue 1, p304
- ISSN
1128-3602
- Publication type
Article