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- Title
The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin–Phycocyanin Mixture on Enterococcus faecalis: The Influence of Different Light Sources.
- Authors
Chiniforush, Nasim; Pourhajibagher, Maryam; Parker, Steven; Benedicenti, Stefano; Bahador, Abbas; Sălăgean, Tudor; Bordea, Ioana Roxana
- Abstract
The purpose of this study was to evaluate the in vitro effect of the chlorophyllin–phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin–phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 ± 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420–480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin–phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity.
- Subjects
LIGHT sources; ENTEROCOCCUS faecalis; PHOTODYNAMIC therapy; SEMICONDUCTOR lasers; MICROBIAL cells; ENTEROCOCCUS
- Publication
Applied Sciences (2076-3417), 2020, Vol 10, Issue 12, p4290
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app10124290