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- Title
Role of galanin receptor 1 in gastric motility in rat.
- Authors
Guerrini, S.; Raybould, H. E.; Anselmi, L.; Agazzi, A.; Cervio, E.; Reeve Jr., J. R.; Tonini, M.; Sternini, C.
- Abstract
Galanin actions are mediated by distinct galanin receptors (GAL-R), GAL-R1, -R2 and -R3. We investigated the role of GAL-R1 in gastric motility and the expression of GAL-R1 in the rat stomach. In vivo, in urethane-anaesthetized rats, galanin (equipotent for all GAL-Rs) induced a short inhibition of gastric motility, followed by increase in tonic and phasic gastric motility; the latter was significantly reduced by the GAL-R1 antagonist, RWJ-57408. Galanin 1–16 (high affinity for GAL-R1 and -R2) induced a long-lasting decrease of intragastric pressure, which was not modified by RWJ-57408. In vitro, galanin and galanin 1–16 induced increase of intragastric pressure that was not affected by RWJ-57408. Tetrodotoxin (TTX) did not suppress the galanin excitatory effect, whereas the effect of galanin 1–16 on gastric contraction was increased by TTX- or N-nitro-L-arginine, an inhibitor of nitric oxide synthase. GAL–R1 immunoreactivity was localized to cholinergic and tachykinergic neurons and to neurons immunoreactive for nitric oxide synthase or vasoactive intestinal polypeptide. This study suggests that an extrinsic GAL-R1 pathway mediates the galanin excitatory action, whereas an extrinsic, non GAL-R1 pathway is likely to mediate the galanin inhibitory effect in vivo. GAL-R1 intrinsic neurons do not appear to play a major role in the control of gastric motility.
- Subjects
GALANIN; GASTROINTESTINAL motility; RATS; NEURONS; GASTROENTEROLOGY; AMINO acids; NEUROPEPTIDES
- Publication
Neurogastroenterology & Motility, 2004, Vol 16, Issue 4, p429
- ISSN
1350-1925
- Publication type
Article
- DOI
10.1111/j.1365-2982.2004.00534.x