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- Title
Establishment of a canine lens epithelial cell line.
- Authors
Akira Matsuda; Yuki Shimizu; Teppei Kanda; Akihiro Ohnishi; Noritaka Maeta; Masahiro Miyabe; Kaori Saeki; Yoshiki Itoh
- Abstract
Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-b) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts.
- Subjects
EPITHELIAL cells; TRANSFORMING growth factors-beta; CELL lines; CRYSTALLINE lens; CELL populations
- Publication
Canadian Journal of Veterinary Research / Revue Canadienne de Recherche Vétérinaire, 2021, Vol 85, Issue 3, p236
- ISSN
0830-9000
- Publication type
Article