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- Title
Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)?carbonyl complex.
- Authors
Waibel, Robert; Alberto, Roger; Willuda, Jörg; Finnern, Ricarda; Schibli, Roger; Stichelberger, Albert; Egli, André; Abram, Ulrich; Mach, Jean-Pierre; Plückthun, Andreas; Schubiger, P. August
- Abstract
We have developed a technetium labeling technology based on a new organometallic chemistry, which involves simple mixing of the novel reagent, a [SUP99m]Tc(I)-carbonyl compound, with a His-tagged recombinant protein. This method obviates the labeling of unpaired engineered cysteines, which frequently create problems in large-scale expression and storage of disulfide-containing proteins. In this study, we labeled antibody single-chain Fv fragments to high specific activities (90 mCi/mg), and the label was very stable to serum and all other challenges tested. The pharmacokinetic characteristics were indistinguishable from iodinated scFv fragments, and thus scFV fragments labeled by the new method will be suitable for biodis-tribution studies. This novel labeling method should be applicable not only to diagnostic imaging with [SUP99m]Tc, but also to radioimmunotherapy approaches with [SUP186/188]Re, and its use can be easily extended to almost any recombinant protein or synthetic peptide.
- Subjects
TECHNETIUM; ORGANOMETALLIC chemistry
- Publication
Nature Biotechnology, 1999, Vol 17, Issue 9, p897
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/12890