We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
There is No Correlation Between Function and Lymphokine Production of HBs--Antigen--Specific Human CD4<sup>+</sup>--Cloned T Cells.
- Authors
Tsutsui, H.; Mizoguchi, Y.; Morisawa, S.
- Abstract
The question whether antigen-specific human CD4+ T cells can be classified on the basis of appropriate and fixed lymphokine production patterns and their corresponding functions still remains to be elucidated. We generated ten CD4+ T-cell clones specific for HBsAg from HbsAb-positive but HBsAg-negative individuals. Seven of these clones exhibited helper activity for HBsAb response, while the three other clones did not. Both helper- and non-helper-type T-cell clones produced interleukin 4 (IL-4) after antigenic stimulation. By stimulation with phytohaem-agglutinin (PHA) plus phorbol myristate acetate (PMA), three of the seven helper-type clones produced interleukin 2(lL-2) in addition to IL-4. However, the other four helper-type clones did not produce IL-2 by such stimulation, although they continued the production of IL-4. All non-helper-type T-cell clones produced a large amount of IL-2, and some of them completely became an IL-2 producer after certain stimulation. These results suggested that both helper- and non-helper-type CD4+ T-cell clones specific for HBsAg might have no strict pattern of lymphokine production as in the TH1/TH2 dichotomy of murine CD4+ T cells. The data also revealed that lymphokine-producing capacity of individual cloned T cells is changeable depending upon the sort of activation.
- Subjects
T cells; LYMPHOCYTES; LYMPHOKINES; PHORBOLS; CLONING; CYTOKINES
- Publication
Scandinavian Journal of Immunology, 1991, Vol 34, Issue 4, p433
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01566.x