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- Title
Expression of Toll-like receptor 2, Dectin-1, and Osteopontin in murine model of pulpitis.
- Authors
Hasan, Arshad; Roome, Talat; Wahid, Mohsin; Ansari, Shazia Akbar; Khan, Javeria Ali; Jilani, Syeda Neha Ahmed; Jawed, Abira; Kiyani, Amber
- Abstract
Objectives: This in vivo animal study aimed to develop a murine model of pulpitis induced by pulp exposure with or without application of zymosan in Naval Medical Research Institute (NMRI) mice and observe expressions of Toll-like receptor (TLR)-2, TLR-4, Dectin-1, Osteopontin (OPN), tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and IL-1ß. Material and methods: A total of 168 NMRI mice were divided into two groups, i.e., group A (n = 84) (pulpitis induced by pulp exposure only) and group B (n = 84) (pulpitis induced by pulp exposure and zymosan application). Right maxillary molar pulps were exposed with ¼ round bur, and animals were sacrificed at 0, 6, 9, 12, 24, 48, and 72 h. The exposed teeth were obtained for real-time polymerase chain reaction (qRT-PCR) analysis and histological and immunohistochemistry (IHC) analysis. Results: Histological evaluation revealed a time-dependent steady increase in inflammation. Similar time-dependent increase in the expression of inflammatory cytokines was noted. Group A exhibited an increase in TLR-4, Dectin-1, and OPN at 6 h, while TLR-2 was expressed at 24 h. Group B expressed TLR-2, Dectin-1, and OPN at 9, 48, and 72 h, respectively (p ≤ 0.05). Expression of OPN and TNF-α exhibited a similar pattern in both groups. IHC also detected expression of TLR-2, Dectin-1, TLR4, and CD68 in some cells at 6 and 9 h. Conclusions: NMRI mice provided for a stable pulp inflammation model. Zymosan may be used to develop pulp inflammation model and study inflammatory response towards fungal antigens. Dental pulp expressed Dectin-1 receptor. OPN and TNF-α exhibited a similar expression pattern. Clinical relevance: Innate immunity of dental pulp is capable of detecting fungal pathogens.
- Subjects
TOLL-like receptors; PULPITIS; OSTEOPONTIN; TUMOR necrosis factors; DENTAL pulp
- Publication
Clinical Oral Investigations, 2023, Vol 27, Issue 3, p1177
- ISSN
1432-6981
- Publication type
Article
- DOI
10.1007/s00784-022-04732-2