We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Genome mining reveals novel biosynthetic gene clusters in entomopathogenic bacteria.
- Authors
Meesil, Wipanee; Muangpat, Paramaporn; Sitthisak, Sutthirat; Rattanarojpong, Triwit; Chantratita, Narisara; Machado, Ricardo A. R.; Shi, Yi-Ming; Bode, Helge B.; Vitta, Apichat; Thanwisai, Aunchalee
- Abstract
The discovery of novel bioactive compounds produced by microorganisms holds significant potential for the development of therapeutics and agrochemicals. In this study, we conducted genome mining to explore the biosynthetic potential of entomopathogenic bacteria belonging to the genera Xenorhabdus and Photorhabdus. By utilizing next-generation sequencing and bioinformatics tools, we identified novel biosynthetic gene clusters (BGCs) in the genomes of the bacteria, specifically plu00736 and plu00747. These clusters were identified as unidentified non-ribosomal peptide synthetase (NRPS) and unidentified type I polyketide synthase (T1PKS) clusters. These BGCs exhibited unique genetic architecture and encoded several putative enzymes and regulatory elements, suggesting its involvement in the synthesis of bioactive secondary metabolites. Furthermore, comparative genome analysis revealed that these BGCs were distinct from previously characterized gene clusters, indicating the potential for the production of novel compounds. Our findings highlighted the importance of genome mining as a powerful approach for the discovery of biosynthetic gene clusters and the identification of novel bioactive compounds. Further investigations involving expression studies and functional characterization of the identified BGCs will provide valuable insights into the biosynthesis and potential applications of these bioactive compounds.
- Subjects
BACTERIAL genomes; GENOMES; METABOLITES; PEPTIDES; GENE clusters; NUCLEOTIDE sequencing; BIOACTIVE compounds; PATHOGENIC bacteria; STREPTOMYCES
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-47121-9