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- Title
Interaction of Chelerythrine with Keyhole Limpet Hemocyanin: a Fluorescence Spectroscopy and Molecular Docking Study.
- Authors
Zhong, M.; Long, R. Q.; Wang, Y. H.; Chen, C. L.
- Abstract
The quenching mechanism between chelerythrine (CHE) and keyhole limpet hemocyanin (KLH) was investigated using fluorescence spectroscopy and molecular docking. The experiments were conducted at three different temperatures (293, 298, and 303 K). The results revealed that the intrinsic fluorescence of KLH was strongly quenched by CHE through a static quenching mechanism. The thermodynamic parameters (ΔG, ΔH, and ΔS) of the interaction were calculated, indicating that the interaction between CHE and KLH was spontaneous and that van der Waals forces and hydrogen bond formation played major roles in the binding process. The intrinsic fluorescence of the tyrosine and tryptophan residues in KLH was studied by synchronous fluorescence, which suggested that CHE changed the conformation of KLH. Finally, molecular docking was used to obtain detailed information on the binding sites and binding affinities between CHE and KLH.
- Subjects
FISSURELLIDAE; FLUORESCENCE spectroscopy; MOLECULAR docking; QUENCHING (Chemistry); HEMOCYANIN
- Publication
Journal of Applied Spectroscopy, 2018, Vol 85, Issue 2, p320
- ISSN
0021-9037
- Publication type
Article
- DOI
10.1007/s10812-018-0651-3