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- Title
Development of technology for purification of plasmid DNAs for pharmaceutical purposes.
- Authors
Volosnikova, E. A.; Akulova, N. I.; Gogina, Ya. S.; Levagina, G. M.; Mikhailova, V. K.; Lebedev, L. R.; Podgornyi, V. F.; Telegina, Yu. V.
- Abstract
Optimization of the stage of fractioning of nucleotide material has been performed, and a technology for purification and obtaining of plasmid DNAs for pharmaceutical purposes has been developed. Plasmid DNAs can be used for construction of DNA vaccines to urgent viral infections. Conditions for extraction and preliminary purification of DNA by salt and alcohol precipitations were determined. An effective gel-filtration procedure of separation of plasmid DNA from contaminants was suggested.
- Subjects
TECHNOLOGY; DNA; DRUGS; VIRUS diseases; GENE therapy
- Publication
Applied Biochemistry & Microbiology, 2010, Vol 46, Issue 9, p865
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S0003683810090085