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- Title
MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB.
- Authors
Wang, Yongxia; Wang, Beixi; Zhou, Hong; Zhang, Xiangnan; Qian, Xinlai; Cui, Jing
- Abstract
Background. Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown. Methods. Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384. Moreover, Spearman's correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC. Results. The expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. The ectopic upregulation of miR-384 significantly suppressed PTC progression, and the inhibition of miR-384 had the opposite effects. Moreover, PRKACB gene was confirmed as the target of miR-384. Conclusion. The study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3′-UTR of PRKACB gene.
- Subjects
ANIMAL experimentation; BIOLOGICAL assay; CELL culture; CELL lines; COLORIMETRY; GENE expression; LYMPH nodes; METASTASIS; POLYMERASE chain reaction; PROTEIN kinases; STATISTICS; THYROID gland tumors; TUMORS; WESTERN immunoblotting; WOUND healing; BIOINFORMATICS; DATA analysis; DISEASE progression; MICRORNA
- Publication
BioMed Research International, 2020, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2020/4983420