We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Expressions of junB and c- fos are enhanced in 4-nitroquinoline 1-oxide-induced rat tongue cancers.
- Authors
Ohyama, Masanobu; Hirayama, Yoshikazu; Tanuma, Jun-ichi; Hirano, Masato; Semba, Ichiro; Shisa, Hayase; Hiai, Hiroshi; Sugihara, Kazumasa; Kitano, Motoo
- Abstract
Activator protein-1 (AP-1) is a transcription factor activated in many tumors. Using 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue cancers (TC), the present study investigated the expression levels of genes that encode the components of AP-1, the jun gene family (c- jun, junB and junD) and the fos gene family (c- fos, fra-1, fra-2 and fosB). Expression levels of junB and c-fos mRNAs in TC were significantly elevated compared with those in epithelial tissue of control rat tongue, although only c- fos mRNA levels tended to be elevated in dysplastic tongue epithelium. Histologically, all 4NQO-induced rat TC were well-differentiated squamous cell carcinomas. Immunostaining for JunB and c-Fos proteins was positive in the nuclei of tumor cells of all TC. It is noteworthy that JunB was negative, but c-Fos was positive in the dysplastic tongue epithelium of the 4NQO-treated rats. Immunostaining for both proteins was negative in tongue mucosal epithelium of control rats. There were no mutations in the coding regions of either junB or c- fos in all the TC examined. These results suggest the possibility that the expressions of junB and c- fos were enhanced stepwise in 4NQO-induced carcinogenesis of rat tongue, and that the coexpression of JunB and c-Fos might play an important role in the establishment of TC.
- Subjects
CARCINOGENESIS; TONGUE cancer; PATHOLOGY; LABORATORY rats; NITROQUINOLINE oxide; GENETICS
- Publication
Pathology International, 2004, Vol 54, Issue 1, p35
- ISSN
1320-5463
- Publication type
Article
- DOI
10.1046/j.1440-1827.2003.01587.x