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- Title
The presence of Turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia.
- Authors
Milošević, Dragana; Ignjatov, Maja; Nikolić, Zorica; Stanković, Ivana; Bulajić, Aleksandra; Marjanović-Jeromela, Ana; Krstić, Branka
- Abstract
A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.
- Subjects
SERBIA; PHYTOPLASMAS; RUTABAGA; CAULIFLOWER mosaic virus; TURNIP mosaic virus; BEET western yellows virus
- Publication
Pesticides & Phytomedicine / Pesticidi i Fitomedicina, 2016, Vol 31, Issue 1/2, p37
- ISSN
1820-3949
- Publication type
Article
- DOI
10.2298/PIF1602037M