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- Title
Feasibility and Diagnostic Accuracy of Saliva-Based SARS-CoV-2 Screening in Educational Settings and Children Aged <12 Years.
- Authors
Hoch, Martin; Vogel, Sebastian; Eberle, Ute; Kolberg, Laura; Gruenthaler, Valerie; Fingerle, Volker; Ackermann, Nikolaus; Sing, Andreas; Liebl, Bernhard; Huebner, Johannes; Kuttiadan, Simone; Rack-Hoch, Anita; Meyer-Buehn, Melanie; Schober, Tilmann; von Both, Ulrich
- Abstract
Children have been disproportionately affected during the COVID-19 pandemic. We aimed to assess a saliva-based algorithm for SARS-CoV-2 testing to be used in schools and childcare institutions under pandemic conditions. A weekly SARS-CoV-2 sentinel study in primary schools, kindergartens, and childcare facilities was conducted over a 12-week-period. In a sub-study covering 7 weeks, 1895 paired oropharyngeal and saliva samples were processed for SARS-CoV-2 rRT-PCR testing in both asymptomatic children (n = 1243) and staff (n = 652). Forty-nine additional concurrent swab and saliva samples were collected from SARS-CoV-2 infected patients (patient cohort). The Salivette® system was used for saliva collection and assessed for feasibility and diagnostic performance. For children, a mean of 1.18 mL saliva could be obtained. Based on results from both cohorts, the Salivette® testing algorithm demonstrated the specificity of 100% (95% CI 99.7–100) and sensitivity of 94.9% (95% CI 81.4–99.1) with oropharyngeal swabs as reference. Agreement between sampling systems was 100% for moderate to high viral load situations (defined as Ct-values <33 from oropharyngeal swabs). Comparative analysis of Ct-values derived from saliva vs. oropharyngeal swabs demonstrated a significant difference (mean 4.23; 95% CI 2.48–6.00). In conclusion, the Salivette® system proved to be an easy-to-use, safe and feasible saliva collection method and a more pleasant alternative to oropharyngeal swabs for SARS-CoV-2 testing in children aged 3 years and above.
- Subjects
SARS-CoV-2; COVID-19 pandemic; VIRAL load; SALIVA; ALGORITHMS
- Publication
Diagnostics (2075-4418), 2021, Vol 11, Issue 10, p1797
- ISSN
2075-4418
- Publication type
Article
- DOI
10.3390/diagnostics11101797