We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Characterization of pTS14, an IncF2:A1:B1 Plasmid Carrying tet (M) in a Salmonella enterica Isolate.
- Authors
Liu, Ying-ying; Liu, Xiao-kang; Cui, Xiao-die; Chen, Min; Li, Shuai-hua; He, Dan-dan; Liu, Jian-hua; Yuan, Li; Hu, Gong-zheng; Pan, Yu-shan
- Abstract
The objective of this study was to explore the genetic and biological features of the tet (M)-harboring plasmid pTS14 in Salmonella enterica strain S14 isolated from a chicken fecal sample. Plasmid pTS14 was identified by conjugation, S1-pulsed-field gel electrophoresis (PFGE), Southern hybridization, and plasmid sequencing. The biological characteristics of pTS14 were assessed via stability, growth kinetics, and starvation survival experiments. Strain S14, belonging to ST3007, harbored a 119-kb tet (M)-bearing IncF2:A1:B1 conjugative plasmid pTS14. The plasmid pTS14 contained a novel transposon Tn 6709 with the genetic structure IS 26 - tnpA1 - tnpA2 -Δ orf13 - LP - tet (M)- tnpX -Δ tnpR -IS 26 , and the resistance genes tet (B), tet (D), strAB , sul2 , and bla TEM–1b. In addition, pTS14 was found to be highly stable in the recipient strain E. coli J53. The transconjugant TS14 exhibited a higher survival ratio than E. coli J53 under permanent starvation-induced stress. The tet (M)-bearing IncF2 epidemic plasmid lineage may accelerate the dissemination of tet (M) and other genes by coselection, which could constitute a potentially serious threat to clinical treatment regimens.
- Subjects
PLASMIDS; SALMONELLA; SALMONELLA enterica; GEL electrophoresis; STARVATION
- Publication
Frontiers in Microbiology, 2020, Vol 11, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2020.01523