We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Proliferation of kuchijirosho causative agent in a fugu-derived cell line.
- Authors
Hashimoto, E.; Miyadai, T.; Ohtani, M.; Kitamura, S.-I.; Oh, M.-J.
- Abstract
Kuchijirosho is a fatal disease of commercially cultured fugu, Takifugu rubripes. The transmissible nature of kuchijirosho strongly suggests that an infectious pathogen is the causative agent. Because it is filtrable, the agent is thought to be a virus; however, it has not yet been identified. The lack of a permissive cell line for the putative kuchijirosho-causing agent (KCA) has hindered research on the identification of this pathogen. We inoculated brain extract prepared from kuchijirosho-affected fugu onto an established fugu cell line, fugu eye, and observed that cytopathic effect appeared 7 days after inoculation. Injection of the culture medium of infected fugu eye cells into fugu resulted in the onset of kuchijirosho, indicating that fugu eye cells are able to proliferate KCA. An infectious fraction separated by sodium iottalamate density gradient centrifugation showed a density of 1.15 g mL−1 equivalent to that of KCA derived from affected fugu brain. To determine whether the genome of KCA is RNA or DNA based, nucleotide synthesis inhibitors were applied to inoculated fugu eye cell line to influence the production of KCA. 5-Fluorouracil but not IUdR showed a concentration-dependent inhibition of KCA yield. These results suggest that KCA is an RNA virus.
- Subjects
FUGU; FISH diseases; PATHOGENIC microorganisms; CELL lines; CELL proliferation; GENOMES; BIOSYNTHESIS; RNA viruses
- Publication
Journal of Fish Diseases, 2008, Vol 31, Issue 6, p443
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1111/j.1365-2761.2008.00925.x