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- Title
Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain.
- Authors
Song, Myungjae; Lim, Jung Min; Min, Seonwoo; Oh, Jeong-Seok; Kim, Dong Young; Woo, Jae-Sung; Nishimasu, Hiroshi; Cho, Sung-Rae; Yoon, Sungroh; Kim, Hyongbum Henry
- Abstract
Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications. While prime editing is a promising technology, PE2 systems often have low efficiency. Here the authors fuse a Rad51 DNA-binding domain to create hyPE2 with improved editing efficiency.
- Subjects
GENOME editing
- Publication
Nature Communications, 2021, Vol 12, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-021-25928-2