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- Title
CD41<sup>+</sup>/CD45<sup>+</sup> Cells Without Acetylcholinesterase Activity Are Immature and a Major Megakaryocytic Population in Murine Bone Marrow.
- Authors
Matsumura-Takeda, Kuniko; Sogo, Shinji; Isakari, Yoshimasa; Harada, Yasuo; Nishioka, Kinue; Kawakami, Takuma; Ono, Toshihide; Taki, Takao
- Abstract
Murine megakaryocytes (MKs) are defined by CD41/CD61 expression and acetylcholinesterase (AChE) activity; however, their stages of differentiation in bone marrow (BM) have not been fully elucidated. In murine lineage-negative (Lin-)/CD45+ BM cells, we found CD41+ MKs without AChE activity (AChE-) except for CD41++ MKs with AChE activity (AChE-), in which CD61 expression was similar to their CD41 level. Lin-/CD41+/CD45+/AChE- MKs could differentiate into AChE+, with an accompanying increase in CD41/CD61 during in vitro culture. Both proplatelet formation (PPF) and platelet (PLT) production for Lin-/CD41+/CD45+/AChE- MKs were observed later than for Lin-/CD41++/CD45+/AChE+ MKs, whereas MK progenitors were scarcely detected in both subpopulations. GeneChip and semiquantitative polymerase chain reaction analyses revealed that the Lin-/CD41+/CD45+/AChE- MKs are assigned at the stage between the progenitor and PPF preparation phases in respect to the many MK/PLT specific gene expressions, including β1-tubulin. In normal mice, the number of Lin-/CD41+/CD45+/AChE- MKs was 100 times higher than that of AChE+ MKs in BM. When MK destruction and consequent thrombocytopenia were caused by an antitumor agent, mitomycin-C, Lin-/CD41+/CD45+/AChE- MKs led to an increase in AChE+ MKs and subsequent PLT recovery with interleukin-11 administration. It was concluded that MKs in murine BM at least in part consist of immature Lin-/CD41+/CD45+/AChE- MKs and more differentiated Lin-/CD41+/CD45++/AChE- MKs. Immature Lin-/CD41+/CD45+/AChE- MKs are a major MK population compared with AChE+ MKs in BM and play an important role in rapid PLT recovery in vivo.
- Subjects
BONE marrow; MEGAKARYOCYTES; BONE marrow cells; ACETYLCHOLINESTERASE; BLOOD platelets; POLYMERASE chain reaction; GENE expression
- Publication
Stem Cells, 2007, Vol 25, Issue 4, p862
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2006-0363