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- Title
Membrane fluidity, membrane lipid pattern, and cytosolic Ca2+ content in platelets from a group of type II diabetic patients with macrovascular complications.
- Authors
Caimi, Gregorio; Presti, Rosalia Lo; Montana, Maria; Canino, Baldassare; Ventimiglia, Giuseppe; Romano, Adele; Catania, Anna; Sarno, Antonio; Caimi, G; Lo Presti, R; Montana, M; Canino, B; Ventimiglia, G; Romano, A; Catania, A; Sarno, A
- Abstract
<bold>Objective: </bold>To evaluate platelet membrane fluidity and some platelet metabolic parameters in type II diabetic patients with macrovascular complications.<bold>Research Design and Methods: </bold>In a group of 21 type II diabetic patients with macrovascular complications, we evaluated platelet membrane fluidity [marking intact resting platelets with the fluorescent probe 1,4-(trimethylamino)-phenyl-4-phenylhexatriene (TMA-DPH)], platelet membrane lipid pattern (cholesterol:phospholipid [C:PL] ratio and individual phospholipids), and platelet cytosolic Ca2+ content (marking intact resting platelets with the fluorescent probe Fura 2AM).<bold>Results: </bold>Platelet membrane fluidity is decreased in type II diabetic patients with macrovascular complications compared with normal subjects (P < 0.001). Platelet membrane C:PL ratio and cytosolic Ca2+ content do not discriminate normal subjects from diabetic patients, and for individual phospholipids, only phosphatidylethanolamine is decreased in diabetic patients compared with control subjects (P = 0.051). In normal subjects, the polarization degree of TMA-DPH is related to phosphatidylserine (P < 0.05) and phosphatidylcholine (P < 0.05), and in diabetic patients the polarization degree of TMA-DPH is related to C:PL ratio (P < 0.05) and sphyngomyelin (P < 0.05).<bold>Conclusions: </bold>In type II diabetic patients with macrovascular complications, we observed an abnormality of platelet membrane fluidity, which may contribute to platelet functional alteration present in this clinical condition.
- Publication
Diabetes Care, 1995, Vol 18, Issue 1, p60
- ISSN
0149-5992
- Publication type
journal article
- DOI
10.2337/diacare.18.1.60