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- Title
Laboratory Performance in Albumin and Total Protein Measurement Using a Commutable Specimen.
- Authors
Lo, Stanley F.; Miller, W. Greg; Doumas, Basil T.
- Abstract
Context.-Discrepant results for serum constituents were observed among peer groups in the College of American Pathologists Comprehensive Chemistry Survey. Objectives.-To assess the performance of serum albumin and total protein measurement procedures and to evaluate the commutability of the conventional survey specimens. Design.-A fresh frozen, off-the-clot serum sample was included along with 4 conventional survey specimens. The fresh frozen, off-the-clot serum sample was prepared in a manner expected to confer commutability with native clinical samples. Results.-For the fresh frozen, off-the-clot serum sample, the mean values for the 17 peer-groups were −0.07 to 0.32 g/dL from the bromocresol green albumin designated comparison method, whereas 4 VITROS (Ortho Clinical Diagnostics, Rochester, New York)peer groups differed by −0.29 to −0.37 g/dL (15 of 21 differences [71%] had P < .001). For bromocresol purple albumin methods, the mean differences from the designated comparison method from 8 peer groups were 0.25 to 0.47 g/dL (all had P < .001). For total protein methods, 23 peer group mean values were −0.07 to 0.15 g/dL from the reference measurement procedure (12 of 24 [50%] had P < .001). The Beckman (Fullerton, California) Synchron LX20 had a bias of −0/30 g/dL (P < .001). The commutability of the conventional method-material combinations (96%) and 13 of 16 bromocresol purple albumin method-materials combinations (81%). All (100%) of the 36 method-material combinations had accessible commutability for total protein. Conclusions.-One (2.2%) of the instrument systems (Synchron) using bromocresol green and none (0%) of the instrument systems using bromocresol purple had satisfactory total-error performance for albumin measurement. Differences in results between bromocresol green and bromocresol purple methods precluded using common reference intervals for interpreting results for serum albumin. Eight of 9 instrument systems (86.5%) had satisfactory total-error performance for total protein measurement.
- Subjects
CALIBRATION; DYES &; dyeing; LONGITUDINAL method; MEDICAL laboratories; PROTEINS; QUALITY assurance; QUALITY control; REFERENCE values; SERUM albumin; STATISTICAL hypothesis testing; T-test (Statistics); COLLEGE of American Pathologists; DATA analysis software; AUTOANALYZERS
- Publication
Archives of Pathology & Laboratory Medicine, 2013, Vol 137, Issue 7, p912
- ISSN
0003-9985
- Publication type
Article
- DOI
10.5858/arpa.2012-0152-CP