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- Title
Rapid-resolution HPLC with spectrometric detection for the determination and identification of isoflavones in soy preparations and plant extracts.
- Authors
Klejdus, B.; Vacek, J.; Benešová, L.; Kopecký, J.; Lapčík, O.; Kubáň, V.
- Abstract
A rapid-resolution HPLC/UV-VIS DAD separation method (which takes <1 min) for the determination and identification of genistin, genistein, daidzein, daidzin, glycitin, glycitein, ononin, formononetin, sissotrin and biochanin A in fmol quantities in submicroliter sample volumes was optimized. A linear gradient elution (0 min 22% B, 1.0 min 80% B, 1.4 min 100% B, 1.8 min 22% B) using a mobile phase containing 0.2 % (v/v) acetic acid (solvent A) and methanol (solvent B) was applied on a Zorbax SB C18 column (1.8 μm particle size) at 80 °C. The method was verified using samples of bits of soy and methanolic extracts from Trifolium pratense, Iresine herbstii and Ononis spinosa plants. Pseudobaptigenin glucoside, irilone, prunetin, texasin, tlatlancuayin and other isoflavones, in addition to aglycones of isoflavones and their β-glucosides and malonyl and acetyl derivatives, were identified by UV-VIS DAD and electrospray mass spectrometric (ESI-MS) detection in the extracts. [Figure not available: see fulltext.]
- Subjects
HIGH performance liquid chromatography; ISOFLAVONES; SOYBEAN; PLANT extracts; BENZOPYRANS
- Publication
Analytical & Bioanalytical Chemistry, 2007, Vol 389, Issue 7/8, p2277
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-007-1606-3