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- Title
The role of MoO<sub>3</sub>NPs on regeneration, growth and development of chrysanthemum cultured in vitro.
- Authors
Nguyen, Phan Le Ha; Thuy, Nguyen Thi Thanh; Mai, Nguyen Thi Nhu; Hoa, Ho Cam Khanh; Khai, Hoang Dac; Tung, Hoang Thanh; Cuong, Do Manh; Luan, Vu Quoc; Phuong, Hoang Thi Nhu; Vinh, Nguyen Quang; Dung, Doan Manh; Nhut, Duong Tan
- Abstract
This study substituted sodium molybdate dehydrate (Na2MoO4.2H2O) in MS medium (Murashige and Skoog 1962) with molybdenum trioxide nanoparticles (MoO3NPs) to evaluate their impact on the morphogenesis, growth, absorption of metal-mineral elements and the activity of antioxidant enzymes of chrysanthemum. The results indicated that 100% callus formation was observed when 1-month-old leaf explants were cultured on medium supplemented with MoO3NPs and Na2MoO4.2H2O in basic MS medium, while leaf explants cultured on MS medium without Na2MoO4.2H2O did not induce callus induction. In addition, the treatment with 223.5 µL/L MoO3NPs resulted in the highest shoot regeneration (33.33%), with 1 shoot per explant, and shoot height (1.14 cm), and fresh weight (1.21 g) compared to those in others and control treatments. Meanwhile, 1-month-old stem node (1 cm) explants cultured on medium supplemented with 149 µL/L MoO3NPs recorded 100% shoot regeneration and the highest number of shoots larger than 2 cm (5 shoots/stem node), shoot height (3.23 cm) and fresh weight (1.87 g) of the shoot cluster after 30 days of culture. During the regeneration stage, the activity of antioxidant enzymes in 149 µg/L MoO3NPs treatment was better than the control (+) treatment (except for SOD) and all the other treatments. Similar results were also observed during the shoot multiplication phase, where either the lack or surplus of Mo in the culture medium also caused the decline of SOD, CAT, and APX enzymes activity. Besides, at the concentration of 6.4 µg/L MoO3NPs in the culture media, nutrients are absorbed more efficiently and rapidly by explants. These findings suggest that substituting ion salt in the culture medium with MoO3NPs led to enhanced absorption, providing a micro-mineral source for plants to support biosynthesis and essential functions. The chrysanthemum plantlets exhibited enhanced rooting and growth when treated with 149 µg/L MoO3NPs, particularly during the rooting stage after 15 days of culture.Key message: Molybdenum trioxide nanoparticles effected on the morphogenesis, growth, absorption of metal-mineral elements and the activity of antioxidant enzymes of chrysanthemum
- Publication
Plant Cell, Tissue & Organ Culture, 2024, Vol 158, Issue 1, p1
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-024-02806-5