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- Title
Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch.
- Authors
Jian-Hua Shao; Yue-Qiu Wang; Xiao-Yan Wu; Rui Jiang; Rong Zhang; Chun-Fu Wu; Jing-Hai Zhang
- Abstract
BmK AS is a β long-chain scorpion peptide from the venom of Buthus martensii Karsch (BmK). It was efficiently expressed as a soluble and functional peptide in Escherichia coli, and purified by metal chelating chromatography. About 4.2 mg/l purified recombinant BmK AS could be obtained. The recombinant BmK AS maintained a similar analgesic activity to the natural one in both the mouse-twisting test and hot-plate procedure. It also exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. BmK AS is the first long-chain scorpion peptide reported to have antimicrobial activity, and is a valuable molecular scaffold for pharmacological research.
- Subjects
ESCHERICHIA coli; ESCHERICHIA; CLONING; PEPTIDES; POISONOUS animals; VENOM; TOXINS; CHROMATOGRAPHIC analysis; ANALYTICAL chemistry
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 1, p23
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-007-9499-y