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- Title
Purification and Properties of a β-1,3-glucanase from Chaetomium sp. that is Involved in Mycoparasitism.
- Authors
Hui Sun; Jinkui Yang; Chao Lin; Xiaowei Huang; Ruihuan Xing; Ke-Qin Zhang
- Abstract
A β-1,3-glucanase was detected, using laminarin as substrate, in the culture broth of Chaetomium sp. Major activity was associated with a 70 kDa protein band visualized on a polyacrylamide gel. β-1,3-Glucanase was purified by a one-step, native gel purification procedure. Optimal activity was observed at pH 6.0 and 30 °C (over 30 min). It could degrade cell walls of plant pathogens including Rhizoctonia solani, Gibberella zeae, Fusarium sp., Colletotrichum gloeosporioides and Phoma sp. The N-terminal amino acid residues of the purified β-1,3-glucanase are PYQLQTP, which do not exhibit homology to other fungal β-1,3-glucanases suggesting it may be a novel enzyme.
- Subjects
CHAETOMIUM; PROTEINS; POLYACRYLAMIDE gel electrophoresis; CELLULASE; CELL membranes; PLANT cell walls; AMINO acids
- Publication
Biotechnology Letters, 2006, Vol 28, Issue 2, p131
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-005-5132-0