We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of 5-trans Isomer of Arachidonic Acid on Model Liposomal Membranes Studied by a Combined Simulation and Experimental Approach.
- Authors
Tremi, Ioanna; Anagnostopoulos, Dimitrios; Spyratou, Ellas; Gkeka, Paraskevi; Georgakilas, Alexandros G.; Chatgilialoglu, Chryssostomos; Cournia, Zoe
- Abstract
Unsaturated fatty acids are found in humans predominantly in the cis configuration. Fatty acids in the trans configuration are primarily the result of human processing (trans fats), but can also be formed endogenously by radical stress. The cis-trans isomerization of fatty acids by free radicals could be connected to several pathologies. Trans fats have been linked to an increased risk of coronary artery disease; however, the reasons for the resulting pathogenesis remain unclear. Here, we investigate the effect of a mono-trans isomer of arachidonic acid (C20:4-5trans, 8cis, 11cis, 14cis) produced by free radicals in physiological concentration on a model erythrocyte membrane using a combined experimental and theoretical approach. Molecular Dynamics (MD) simulations of two model lipid bilayers containing arachidonic acid and its 5-trans isomer in 3 mol% were carried out for this purpose. The 5-trans isomer formation in the phospholipids was catalyzed by HOCH2CH2S· radicals, generated from the corresponding thiol by γ-irradiation, in multilamellar vesicles of SAPC. Large unilamellar vesicles were made by the extrusion method (LUVET) as a biomimetic model for cis-trans isomerization. Atomic Force Microscopy and Dynamic Light Scattering were used to measure the average size, morphology, and the z-potential of the liposomes. Both results from MD simulations and experiments are in agreement and indicate that the two model membranes display different physicochemical properties in that the bilayers containing the trans fatty acids were more ordered and more rigid than those containing solely the cis arachidonic acid. Correspondingly, the average size of the liposomes containing trans isomers was smaller than the ones without.
- Subjects
ARACHIDONIC acid; LIPOSOMES; UNSATURATED fatty acids; CIS-trans isomers; ERYTHROCYTE membranes
- Publication
Journal of Membrane Biology, 2018, Vol 251, Issue 3, p475
- ISSN
0022-2631
- Publication type
journal article
- DOI
10.1007/s00232-018-0029-8