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- Title
Effect of storage conditions on SARSCoV-2 RNA quantification in wastewater solids.
- Authors
Simpson, Adrian; Topol, Aaron; White, Bradley J.; Wolfe, Marlene K.; Wigginton, Krista R.; Boehm, Alexandria B.
- Abstract
SARS-CoV-2 RNA in wastewater settled solids is associated with COVID-19 incidence in sewersheds and therefore, there is a strong interest in using these measurements to augment traditional disease surveillance methods. A wastewater surveillance program should provide rapid turn around for sample measurements (ideally within 24 hours), but storage of samples is necessary for a variety of reasons including biobanking. Here we investigate how storage of wastewater solids at 4 °C, −20 °C, and −80 ◦C affects measured concentrations of SARS-CoV-2 RNA. We find that short term (7 or 8 d) storage of raw solids at 4 °C has little effect on measured concentrations of SARS-CoV-2 RNA, whereas longer term storage at 4 °C (35–122 d) or freezing reduces measurements by 60%, on average. We show that normalizing SARS-CoV-2 RNA concentrations by concentrations of pepper mild mottle virus (PMMoV) RNA, an endogenous wastewater virus, can correct for changes during storage as storage can have a similar effect on PMMoV RNA as on SARS-CoV-2 RNA. The reductions in SARS-CoV-2 RNA in solids during freeze thaws is less than those reported for the same target in liquid influent by several authors.
- Subjects
RNA; SEWAGE; SARS-CoV-2; COVID-19; SOLIDS; SEWAGE purification; VIRUSES
- Publication
PeerJ, 2021, p1
- ISSN
2167-8359
- Publication type
Article
- DOI
10.7717/peerj.11933